Document 0506
 DOCN  M9480506
 TI    The intracytoplasmic domain of gp41 mediates polarized budding of human
       immunodeficiency virus type 1 in MDCK cells.
 DT    9410
 AU    Lodge R; Gottlinger H; Gabuzda D; Cohen EA; Lemay G; Departement de
       Microbiologie et Immunologie, Universite de; Montreal, Quebec, Canada.
 SO    J Virol. 1994 Aug;68(8):4857-61. Unique Identifier : AIDSLINE
       MED/94309148
 AB    Human immunodeficiency virus type 1 (HIV-1) has been shown to exhibit a
       specific basolateral release in polarized epithelial cells. Previous
       investigators have used vaccinia virus recombinants expressing HIV
       proteins to demonstrate that virus release is nonpolarized in the
       absence of viral envelope glycoproteins. In this study, we developed a
       transient expression system which allows the use of Madin-Darby canine
       kidney polarized epithelial cells directly grown on semipermeable
       membranes. This procedure allowed us to investigate polarized HIV viral
       budding following introduction of proviral DNA constructs. Expression of
       env gene products in trans demonstrated the ability to polarize
       env-negative viruses in a dose-dependent manner. The targeting signal
       for polarized virus release was shown to be present in the envelope gp41
       transmembrane protein and absent from the gp120 portion of env. At least
       part of this signal is within the gp41 intracytoplasmic domain. Mutants
       of the p17gag matrix protein were shown to be nonpolarized only when
       unable to interact with the envelope glycoproteins. Together, these data
       are consistent with a model of polarized virus budding in which capsid
       proteins, lacking a targeting signal, are targeted for specific
       basolateral release via an interaction of p17 with the envelope
       glycoprotein containing the polarization signal in its intracytoplasmic
       domain.
 DE    Animal  Biological Transport  Cell Line  Cloning, Molecular
       Cytoplasm/METABOLISM  Dogs  Gene Products, env/PHYSIOLOGY  Gene
       Products, gag/GENETICS/METABOLISM  HIV Antigens/GENETICS/METABOLISM  HIV
       Envelope Protein gp41/GENETICS/*PHYSIOLOGY  HIV-1/*PHYSIOLOGY  Mutation
       Protein Precursors/PHYSIOLOGY  Signal Peptides/METABOLISM  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Virus Replication  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).